Composite

Part:BBa_M31415:Design

Designed by: Stephanie Nix   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2007-02-26)


20.109 refactor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1908
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1908
    Illegal NheI site found at 1734
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1908
    Illegal BamHI site found at 2869
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1908
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1908
    Illegal AgeI site found at 1225
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Homologous recombination, redesigning existing genes to get rid of existing overlap.


Source

M13KO7: 8668-2225

References